Xanthomonas hortorum pv. pelargonii (Xhp) - DAS ELISA                           Home    Products    Testing Services   Supplies

Catalog #:  B109
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This ELISA reacted with all Xcp isolates tested.  Reaction of this test is strong.  Optical Density at 405nm  is in a range of 1.200 - 3.000 depending on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:   This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. citri,  X. c. pv. dieffenbachiae, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  This ELISA shows cross-reaction with Xanthomonas campestris pv. armoraciae and some cross-reaction (16%) with X. c. pv. campestris  
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Xcp in petioles, stems, leaves and roots of infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

B109-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

B109-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

B109-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
B 109-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
B109-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
B109-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

B109-C1

Coating Antibody

500

125

2 lb (1 kg)

B109-C2

Coating Antibody

1000

235

2 lb (1 kg)

B109-C3

Coating Antibody

5000

805

2 lb (1 kg)

B109-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

B109-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

B109-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
BS109-LF1 ASPOT√CHECK LF™, lateral flow device (Dip Strip), complete kit for real-time quick assay 10 120 2 lb (1 kg)
BS109-LF2 ASPOT√CHECK LF™, lateral flow device (Dip Strip), complete kit for real-time quick assay 100 1080 13 lb (6 kg)

B109-P1

Positive control

18

18

1 lb (0.5 kg)

B109-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Bacterium

Name:  Xanthomonas campestris pv. pelargonii 
Acronym:  Xcp
Synonyms: 
Group/Genus:  Xanthomonas

Host plants and Diseases:   Bacterial blight of geranium
Symptoms:  Leaf blight, plant wilting.

Bacterial Transmission:   Vegetative propagation.
Bacterial Infection:  Systemic infection in geranium plant.

Reference:


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