Catalog #: B110
Reactivity: This ELISA reacted with all Xcph isolates tested. Reaction of this test is moderately strong. Optical Density at 405nm is in a range of 0.400 - 1.600 depending on the bacterial titers in the samples tested.
Sensitivity: Sensitivity of the ELISA is relatively high. The bacterium can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.
Specificity: This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. dieffenbachiae, and X. c. pv. vesicatoria. There is no cross reaction with healthy plant tissues. Background is very low on all of the negative control wells.
Application: The test can be used to detect Xcph in infected host plants as well as in pure bacterial cultures.
It is easy and convenient To Place
Information About the Bacterium
Name: Xanthomonas campestris pv. phaseoli
Host plants and Diseases: Bacterial blight of beans
Bacterial Transmission: Seeds
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