Xanthomonas campestris pv. phaseoli (Xcph) - DAS ELISA                     Home    Products    Testing Services   Supplies

Catalog #:  B110
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                              

Reactivity:  This ELISA reacted with all Xcph isolates tested.  Reaction of this test is moderately strong.  Optical Density at 405nm  is in a range of 0.400 - 1.600 depending on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Specificity:   This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri,  X. c. pv. dieffenbachiae, and X. c. pv. vesicatoria.  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Xcph in infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

B110-R1

ELISA Reagents, Alkaline Phosphatase

500

220

2 lb (1 kg)

B110-R2

ELISA Reagents, Alkaline Phosphatase

1000

385

3 lb (1.5 kg)

B110-R3

ELISA Reagents, Alkaline Phosphatase

5000

1515

12 lb (6 kg)

B110-R4

ELISA Reagents, Alkaline Phosphatase

100

85

12 lb (6 kg)
B110-K1 ELISA Kit, Alkaline Phosphatase

500

340

2 lb (1 kg)
B110-K2 ELISA Kit, Alkaline Phosphatase

1000

530

3 lb (1.5 kg)

B110-C1

Coating Antibody

500

110

2 lb (1 kg)

B110-C2

Coating Antibody

1000

195

2 lb (1 kg)

B110-C3

Coating Antibody

5000

690

2 lb (1 kg)

B110-D1

Detecting conjugate, Alkaline Phosphatase

500

135

2 lb (1 kg)

B110-D2

Detecting conjugate, Alkaline Phosphatase

1000

215

2 lb (1 kg)

B110-D3

Detecting conjugate, Alkaline Phosphatase

5000

860

2 lb (1 kg)

B110-P1

Positive control

18

15

1 lb (0.5 kg)

B110-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Bacterium

Name:  Xanthomonas campestris pv. phaseoli
Acronym:  Xcph
Synonyms: 
Group/Genus:  Xanthomonas

Host plants and Diseases:  Bacterial blight of beans
Symptoms:  Leaf blight.  Yellow or chlorotic form of irregularly shaped lesions.

Bacterial Transmission:  Seeds
Bacterial Infection:  Infection occurs on leaves, stems, and seeds.  Seed-borne bacteria is the primary way of infection.  The secondary infection is caused by rain splash. 

Reference:


 

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