Phytophthora (Phyt) - TAS ELISA                                                         Home    Products    Testing Services   Supplies

Catalog #:  F111
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format:  TAS ELISA                              

Reactivity:   This ELISA detects at least the following 16 Phytophthora species: P. cactorum, P. cambivora, P. capsici, P. cinnamomi, P. citricola, P. citrophthora, P. cryptogea, P. drechsleri, P. erythroseptica, P. fragariae, P. infestans, P. megasperma, P. palmivora, P. parasitica, and P. sojae.  It reacted with all 39 isolates of Phytophthora infestans tested.

Reaction of this test is moderately strong.  Optical Density at 405nm  is in a range of 0.480 - 1.800 depending on the fungal titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderately high.  The fungus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Specificity:   This test system reacts negatively with the following species of fungi:  Pythium aphanidermatum, P. catenulatum, P. heterothalicum, P. irregulare, P. oligandrum, P. spinosum, P. splendens, P. torulosum, P. ultimum, P. ultimum var. ultimum.  The test did not react with isolates of Aspergillussp., Fusarium sp., Monilinia sp., Penicilliumsp., Rhizoctonia sp., and Sclerotiniasp.  There are moderate cross-reaction occurring with pure culture extracts of Pythium aristosporum, P. arrhenomanes, P. graminicola, P. paroecandrum, and P. violae, and weak cross-reaction occurring with pure culture extracts of Pythium multisporum, P. myriotylum, P. sylvaticum, P. tardicrescens, P. vanterpoolii.

There is no cross reaction with healthy plant tissues.  Background is low on all of the negative control wells.

Application:  The test can be used to detect the fungi in many host plant tissues infected with Phytophthora spp., such as leaves, stems, roots, seeds, and potato tubers, as well as in pure fungal cultures.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

F111-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

F111-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

F111-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
F111-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
F111-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
F111-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

F111-C1

Coating Antibody

500

125

2 lb (1 kg)

F111-C2

Coating Antibody

1000

235

2 lb (1 kg)

F111-C3

Coating Antibody

5000

805

2 lb (1 kg)

F111-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

F111-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

F111-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
FS06-AGR AGRISCREEN®, qualitative and semi-quantitative test kit

96

440

2 lb (1 kg)
FS06-LF1
10 145 2 lb (1 kg)
FS06-LF2 ALERT-LF™, lateral flow device, complete kit for real-time quick assay 100 1140 13 lb (6 kg)

F111-P1

Positive control

18

18

1 lb (0.5 kg)

F111-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000  400
6 lb (3.0 kg)

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To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Fungus

Name:  Phytophthora
Acronym:  Phyt
Synonyms: 
Group/Genus:  Phytophthora

Transmission:  Air-born sporangia, infected plant tissues, seed, tuber, soil and agricultural activities.
Main host plants:  Wide range of host plants including many important crops.

Fungal Structures: Sporangia, Sporangial spores, Mycelia.
Fungal Infection:  The fungi infect leaves, stems, roots, tubers and other organs of host plants.  The infected plant shows various symptoms including leaf lesions, stem cankers, root and tuber rotting, or the death of whole plant. 

Reference: