Dried ControlsFor ELISA Reagents
Storage and usage
Store controls at 4 oC. For long term storage, controls should
be kept at -20 oC.
Controls are recommended for ELISA tests with AC Diagnostics’s Reagents only. Positive
controls may contain more than one virus.
Use the sample extraction buffer for the test system and protocol in which
the control will be used to prepare the control. The volume on the control label
is the amount of sample extraction buffer required to prepare the control.
To prepare the control, pour out all of the contents from the tube and grind
it with this amount of sample extraction buffer by a mortar and pestle, or other
grinding devices. Positive control must be grounded very well to make sure that
plant tissue be crushed to release virus.
The prepared control can be used immediately, or divided into aliquots and
stored frozen ( -20 oC). Each aliquot should be sufficient for at
least one use. For example, if you use this control in one well each time
you run the test, prepare 120 ul aliquots. Prepare 220 ul aliquots if you
use the control in two wells.
Control aliquots must be kept frozen until just before use. Do not refreeze
controls once they've been thawed.
At the time you run the test, remove one control from storage and allow it to
thaw. Add the prepared control to the appropriate control well at the same
volume of the sample.
If you have any questions about preparing and using controls, please contact AC Diagnostics, Inc.