Tomato Yellow Leaf Curl Virus (TYLCV) - TAS ELISA                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V257
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:                                   

Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.900 - 1.800 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is very low on the negative control wells.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V257-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V257-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V257-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V257-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V257-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V257-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V257-C1

Coating Antibody

500

125

2 lb (1 kg)

V257-C2

Coating Antibody

1000

235

2 lb (1 kg)

V257-C3

Coating Antibody

5000

805

2 lb (1 kg)

V257-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V257-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V257-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V257-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V257-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
 Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Tomato Yellow Leaf Curl Bigenminivirus
Acronym:  TYLCV
Synonyms:  tomato leaf curl virus.
Genus: 

Strains:      
Transmission: Transmitted by a vector; an insect; Bemisia tabaci; Aleyrodidae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission; transmitted by mechanical inoculation (poorly); transmitted by grafting; not transmitted by contact between plants.
 

Geographical Distribution: Spreads in Cyprus, India, Iraq, Israel, Jordan, Lebanon, Nigeria, Senegal, Sudan, Thailand, Tunisia, and Turkey (Verma et al., 1975; Lana and Wilson, 1976).
Diagnostically Susceptible Species and Symptoms: 
Lycopersicon esculentum - severe systemic leaf chlorosis and malformation.
Datura stramonium - interveinal leaf chlorosis.
Nicotiana glutinosa, N. benthamiana - systemic vein clearing, then downward curling of the leaf and its margins.
Phaseolus vulgaris - interveinal chlorosis, downward curling of the leaves.

References:
Cherif, C. and Russo, M. (1983). Phytopath. Z. 108: 221.
Ioannou, N. (1987). Pl. Path 36: 367.
 


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