Tobacco Mild Green Mosaic Virus (TMGMV) - DAS ELISA                                       Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V244
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA                                

Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.700 - 1.800 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is low on the negative control wells.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V244-R1 ELISA Reagents, Alkaline Phosphatase

500

280

2 lb (1 kg)
V244-R2 ELISA Reagents, Alkaline Phosphatase

1000

495

3 lb (1.5 kg)
V244-R3 ELISA Reagents, Alkaline Phosphatase

5000

1985

12 lb (6 kg)
V244-R4 ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)
V244-K1 ELISA Kit, Alkaline Phosphatase

500

425

2 lb (1 kg)
V244-K2 ELISA Kit, Alkaline Phosphatase

1000

650

3 lb (1.5 kg)
V244-C1 Coating Antibody

500

140

2 lb (1 kg)
V244-C2 Coating Antibody

1000

255

2 lb (1 kg)
V244-C3 Coating Antibody

5000

885

2 lb (1 kg)
V244-D1 Detecting conjugate, Alkaline Phosphatase

500

175

2 lb (1 kg)
V244-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V244-D3 Detecting conjugate, Alkaline Phosphatase

5000

1115

2 lb (1 kg)
V244-P2 ELISA control, positive

18

18

1 lb (0.5 kg)
V244-N1 Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Tobacco Mild Green Motaic Virus
Acronym:  TMGMV
Synonyms:
Genus: tobamovirus

Strains:       
Transmission: Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.

Geographical Distribution: Probably distributed worldwide (in Nicotiana glauca in Mediterranean climatic regions).
Diagnostically Susceptible Species and Symptoms: Nicotiana glutinosa, N. sylvestris, N. tabacum cvs Xanthi-nc, White Burley - systemic yellow mosaic, ringspots. Datura stramonium, Chenopodium amaranticolor - local lesions; not systemic. Eryngium aquaticum, E. planum - systemic yellow flecks. Lycopersicon esculentum cv. Rutgers - yellow mosaic with Australian isolate.
References:
Van de Walle, M.J. and Siegel, A. (1982). Phytopathology 72: 390.
Wetter, C. (1980). Z. PflKrankh. PflPath. PflSchutz. 87: 150.
Wetter, C. (1984). Phytopathology 74: 1308.
Wetter, C. (1986). In: Plant Viruses; Vol. 2, The Rod-Shaped Plant Viruses; eds M.H.V. van Regenmortel and H. Fraenkel-Conrat. Plenum Press, New York.
Wetter, C. (1989). CMI/AAB Descr. Pl. Viruses No. 351, 6 pp.


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