Squash Leaf Curl Bigeminivirus (SLCV) - DAS ELISA                               Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V256
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:                                   

Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.800 - 1.800 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is very low on the negative control wells.

Products: 

Catalog Number

Description

Test Wells

Price $USD Ship Weight
(Estimated)

V256-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V256-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V256-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V256-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V256-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V256-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V256-C1

Coating Antibody

500

125

2 lb (1 kg)

V256-C2

Coating Antibody

1000

235

2 lb (1 kg)

V256-C3

Coating Antibody

5000

805

2 lb (1 kg)

V256-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V256-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V256-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V256-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V256-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

*IDENTIKIT™ offers the quantitative or semi-quantitative detection of a chosen pathogen.

It is easy and convenient:
To Place An Order 

To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Squash Leaf Curl Bigeminivirus
Acronym:  SLCV
Synonyms: probably muskmelon necrotic mosaic virus.
Genus: 

Strains:       
Transmission: Transmitted by a vector; an insect; Bemisia tabaci; Aleyrodidae. Transmitted in a persistent manner. Virus not transmitted by mechanical inoculation.

Geographical Distribution: Spreads in the USA (California).
Diagnostically Susceptible Species and Symptoms: 
Cucurbita pepo, Cucurbita maxima, Cucurbita moschata - severe stunting and leaf curling.
Phaseolus vulgaris - green mosaic leaf symptoms and deformation.

References:
Cohen, S., Duffus, J.E., Larsen, R.C., Liu, H.Y. and Flock, R.A. (1983). Phytopathology 73: 1669.

 


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