Maize Rough Dwarf Fijivirus (MRDV)                                            Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  VS32
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:                                   

Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.800 - 1.900 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is very low on the negative control wells.

Products: 

Catalog Number

Description Test Wells Price $US Ship Weight
(Estimated)
VS32-P1 Positive control 10 40 1 lb (0.5 kg)
VS32-N1 Negative control 10 40 1 lb (0.5 kg)

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Maize Rough Dwarf Fijivirus
Acronym:  MRDV
Synonyms:  virus del nanismo ruvido del mais.
Genus: 

Strains: type strain (TS), cereal tillering disease strain (CTD) (Boccardo and Milne, 1984; Lindsten and Gerhardson, 1971), mal de Rio Cuarto (MR4) (Boccardo and Milne, 1984; Nome et al., 1981).
Transmission: Transmitted by a vector; an insect; Delphacodes propinqua, Dicranotropis hamata, Laodelphax striatellus, Javasella pellucida, Sogatella vibix; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; transmitted congenitally to the progeny of the vector (one unconfirmed report); transmitted by mechanical inoculation (injection; but very inefficient); not transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical Distribution: Spreads in Argentina, the former Czechoslovakia, France, Israel, Italy, Norway, Spain, Sweden, and the former Yugoslavia.
Diagnostically Susceptible Species and Symptoms: 
Hordeum vulgare cv. Delesa - stunting.
Zea mays American yellow dent hybrids; Wisconsin 641 AA - stunting, dark green colour, pale yellow enations on abaxial surface of leaves and leaf sheaths.

References:
Francki, R.I.B. and Boccardo, G. (1983). In: The Reoviridae. p. 155; ed. K.W. Joklik. Plenum Press, New York.
Francki, R.I.B., Milne, R.G. and Hatta, T. (1984). In: An Atlas of Plant Viruses. Vol. 1. CRC Press, Boca Raton, Florida.
 


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