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                                                  PRODUCT ITEMS Immunocapture PCR Kits       


1.  Components of Immunocapture PCR Kits

Coated PCR Tubes/Plates

1x8 PCR strips pre-coated with specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb)

Sample Buffer

PCR sample extraction buffer, in 5x concentrate liquid or in dry powder 

Washing Buffer

PCR plate washing buffer, in 20x concentrate liquid or in dry powder 

Primers

Forward and reverse PCR or RT-PCR primers, in form of freezing-dried powder

Master Mix

Master Mix, including RT-PCR buffer, dNTPs, Mg++, dH2O and/or RNasin

Enzymes

Reverse transcriptase,  Taq polymerase

Positive Controls

Positive control made by virus infected material or by viral cDNA template, in freezing-dried powder

Negative Control

Negative control made by healthy plant material, in freezing-dried powder

Instruction

Specific test instructions for each of the three immunocapture PCR kits  

2.  Kit Formats

Component ACDcap Immunocapture Kit for PCR Assay ACDamp Immunocapture PCR Kit ACDcom Complete Immunocapture PCR  Kit
Coated PCR Tubes/Plates Yes Yes Yes
PCR Sample Buffer Yes Yes Yes
PCR tube Washing Buffer Yes Yes Yes
PCR or RT-PCR Primers   Yes Yes
Master Mix     Yes
Enzymes     Yes
Positive Controls-virus Yes Yes Yes
Positive Controls-cDNA     Yes
Negative Control Yes Yes Yes
Instruction Yes Yes Yes

3.  Contents of Immunocapture PCR Kits

    ACDcap Immunocapture Kit for PCR Assay, Tests Instruction

No

Item

48 Tests

96 Tests

288 Tests

Storage

1

1X8 PCR strips coated with capturing antibody

6 strips

12 strips

36 strips

2-6oC

2

PCR Sample Buffer, powder

4.6 g

11.6 g

23.2 g

2-6oC

3

Washing Buffer, powder

9.6 g

19.2 g

57.3 g

2-6oC

4

Tween-20, for sample and washing buffers

2.5 g

6.0 g

13.0 g

2-6oC

5

Positive Controls-virus, 1.0 ml/bottle

1 bottle

1 bottle

2 bottles

2-6oC

6

Negative Control, 1.0 ml/bottle

1 bottle

1 bottle

2 bottles

2-6oC

7

Instruction

1

1

1

Room temperature

 ACDamp Immunocapture PCR Kit, Tests Instruction

No

Item

48 Tests

96 Tests

288 Tests

Storage

1

1X8 PCR strips coated with capturing antibody

6 strips

12 strips

36 strips

2-6oC

2

PCR or RT-PCR primers, freezing-dried

1 vial

1 vial

3 vials

2-6oC

3

PCR Sample Buffer, powder

4.6 g

11.6 g

23.2 g

2-6oC

4

Washing Buffer, powder

9.6 g

19.2 g

57.3 g

2-6oC

5

Tween-20, for sample and washing buffers

2.5 g

6.5 g

13.0 g

2-6oC

6

Positive Controls-virus, 1.0 ml/bottle

1 bottle

1 bottle

2 bottles

2-6oC

7

Negative Control, 1.0 ml/bottle

1 bottle

1 bottle

2 bottles

2-6oC

8

Instruction

1

1

1

Room temperature

 ACDcom Complete Immunocapture PCR Kit, Tests Instruction

No

Item

48 Tests

96 Tests

288 Tests

Storage

1

1X8 PCR strips coated with capturing antibody

6 strips

12 strips

36 strips

2-6oC

2

PCR or RT-PCR primers, freezing-dried

1 vial

1 vial

3 vials

2-6oC

3

Master Mix

1 vial

1 vial

3 vials

-20oC

4

Reverse transcriptase and/or Taq polymerase

1 vial

1 vial

1 vial

-20oC

5

PCR Sample Buffer, powder

4.6 g

11.6 g

23.2 g

2-6oC

6

Washing Buffer, powder

9.6 g

19.2 g

57.3 g

2-6oC

7

Tween-20, for sample and washing buffers

2.5 g

6.5 g

13.0 g

2-6oC

8

Positive Controls-virus, 1.0 ml/bottle

1 bottle

1 bottle

2 bottles

2-6oC

9

Positive Controls-cDNA

1 bottle

1 bottle

2 bottles

2-6oC

10

Negative Control, 1.0 ml/bottle

1 bottle

1 bottle

2 bottles

2-6oC

11

Instruction

1

1

1

Room temperature


 


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