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PRODUCT ITEMS – Immunocapture PCR Kits
1.
Components of Immunocapture PCR Kits
|
Coated PCR Tubes/Plates |
1x8 PCR strips pre-coated with specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) |
|
Sample Buffer |
PCR sample extraction buffer, in 5x concentrate liquid or in dry powder |
|
Washing Buffer |
PCR plate washing buffer, in 20x concentrate liquid or in dry powder |
|
Primers |
Forward and reverse PCR or RT-PCR primers, in form of freezing-dried powder |
|
Master Mix |
Master Mix, including RT-PCR buffer, dNTPs, Mg++, dH2O and/or RNasin |
|
Enzymes |
Reverse transcriptase, Taq polymerase |
|
Positive Controls |
Positive control made by virus infected material or by viral cDNA template, in freezing-dried powder |
|
Negative Control |
Negative control made by healthy plant material, in freezing-dried powder |
|
Instruction |
Specific test instructions for each of the three immunocapture PCR kits |
2. Kit Formats
| Component | ACDcap Immunocapture Kit for PCR Assay | ACDamp Immunocapture PCR Kit | ACDcom Complete Immunocapture PCR Kit |
| Coated PCR Tubes/Plates | Yes | Yes | Yes |
| PCR Sample Buffer | Yes | Yes | Yes |
| PCR tube Washing Buffer | Yes | Yes | Yes |
| PCR or RT-PCR Primers | Yes | Yes | |
| Master Mix | Yes | ||
| Enzymes | Yes | ||
| Positive Controls-virus | Yes | Yes | Yes |
| Positive Controls-cDNA | Yes | ||
| Negative Control | Yes | Yes | Yes |
| Instruction | Yes | Yes | Yes |
3. Contents of Immunocapture PCR Kits
ACDcap Immunocapture Kit for PCR Assay, Tests Instruction
|
No |
Item |
48 Tests |
96 Tests |
288 Tests |
Storage |
|
1 |
1X8 PCR strips coated with capturing antibody |
6 strips |
12 strips |
36 strips |
2-6oC |
|
2 |
PCR Sample Buffer, powder |
4.6 g |
11.6 g |
23.2 g |
2-6oC |
|
3 |
Washing Buffer, powder |
9.6 g |
19.2 g |
57.3 g |
2-6oC |
|
4 |
Tween-20, for sample and washing buffers |
2.5 g |
6.0 g |
13.0 g |
2-6oC |
|
5 |
Positive Controls-virus, 1.0 ml/bottle |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
6 |
Negative Control, 1.0 ml/bottle |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
7 |
Instruction |
1 |
1 |
1 |
Room temperature |
ACDamp Immunocapture PCR Kit, Tests Instruction
|
No |
Item |
48 Tests |
96 Tests |
288 Tests |
Storage |
|
1 |
1X8 PCR strips coated with capturing antibody |
6 strips |
12 strips |
36 strips |
2-6oC |
|
2 |
PCR or RT-PCR primers, freezing-dried |
1 vial |
1 vial |
3 vials |
2-6oC |
|
3 |
PCR Sample Buffer, powder |
4.6 g |
11.6 g |
23.2 g |
2-6oC |
|
4 |
Washing Buffer, powder |
9.6 g |
19.2 g |
57.3 g |
2-6oC |
|
5 |
Tween-20, for sample and washing buffers |
2.5 g |
6.5 g |
13.0 g |
2-6oC |
|
6 |
Positive Controls-virus, 1.0 ml/bottle |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
7 |
Negative Control, 1.0 ml/bottle |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
8 |
Instruction |
1 |
1 |
1 |
Room temperature |
ACDcom Complete Immunocapture PCR Kit, Tests Instruction
|
No |
Item |
48 Tests |
96 Tests |
288 Tests |
Storage |
|
1 |
1X8 PCR strips coated with capturing antibody |
6 strips |
12 strips |
36 strips |
2-6oC |
|
2 |
PCR or RT-PCR primers, freezing-dried |
1 vial |
1 vial |
3 vials |
2-6oC |
|
3 |
Master Mix |
1 vial |
1 vial |
3 vials |
-20oC |
|
4 |
Reverse transcriptase and/or Taq polymerase |
1 vial |
1 vial |
1 vial |
-20oC |
|
5 |
PCR Sample Buffer, powder |
4.6 g |
11.6 g |
23.2 g |
2-6oC |
|
6 |
Washing Buffer, powder |
9.6 g |
19.2 g |
57.3 g |
2-6oC |
|
7 |
Tween-20, for sample and washing buffers |
2.5 g |
6.5 g |
13.0 g |
2-6oC |
|
8 |
Positive Controls-virus, 1.0 ml/bottle |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
9 |
Positive Controls-cDNA |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
10 |
Negative Control, 1.0 ml/bottle |
1 bottle |
1 bottle |
2 bottles |
2-6oC |
|
11 |
Instruction |
1 |
1 |
1 |
Room temperature |