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PRODUCT ITEMS - PathogenID Test Kit       

1.  Components of PathogenID Test Kit

Coated Plates

96-well plates coated with specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) Empty plates for indirect ELISA

Detecting Conjugate Alkaline Phosphatase, Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate. Enzyme conjugate is 1x solution and ready to use.
BluePhos Substrate Part A and Part B, 1x. BluePhos substrate includes two parts of 1x solutions and is ready to use.  Mix equal volumes of Part A and Part B in a container immediately before use.
Controls Positive control and negative control,  18 test wells per battle.
Buffers PBST Washing Buffer (powder), Tween-20 (for PBST and sample buffer), *SB1 General Sample buffer (powder), *SB4 grape sample buffer (powder), *SB5 blueberry sample buffer (powder) (*Only one of the three buffers is included in DAS and TAS ELISA)
Instruction Specific test instructions for each of the three different PathogenID Formats.  

2.  Contents of PathogenID

        DAS ELISA, alkaline phosphatase conjugate, test instruction

Item

96 wells

192 wells

384 wells

Storage

96-well plates coated with antibody

1

2

4

2-6oC

Detecting conjugate, alkaline phosphatase, 1x

12 ml

23 ml

45 ml

2-6oC

BluePhos substrate, Part A and Part B, 1x

A: 6ml; B: 6ml

A: 11ml; B: 11ml

A: 22ml; B: 22ml

2-6oC

ELISA control, positive, 1.8 ml/bottle

1

1

2

2-6oC

ELISA control, negative, 1.8 ml/bottle

1

1

2

2-6oC

PBST Washing buffer, powder

14.4 g

28.7 g

57.3 g

Room temperature

Tween-20, for PBST and sample buffer

4.0 g

7.0 g

14.0 g

Room temperature

SB1 General Sample buffer, powder*

5.8 g

11.6 g

23.2 g

Room temperature

SB4 grape sample buffer, powder*

24.7 g

49.4 g

98.7 g

Room temperature

SB5 blueberry sample buffer, powder*

8.6 g

17.3 g

34.5 g

Room temperature

Instruction

1

1

1

 

                                             **Only one of the three buffers is included

        TAS ELISA, alkaline phosphatase conjugate, test instruction

Item

96 wells

192 wells

384 wells

Storage

96-well plates coated with antibody

1

2

4

2-6oC

Detecting conjugate, alkaline phosphatase, Bottle A & Battle B, 1x

A: 6ml; B: 6ml

A: 12ml; B: 12ml

A: 23ml; B: 23ml

2-6oC

BluePhos substrate, Part A and Part B, 1x

A: 6ml; B: 6ml

A: 11ml; B: 11ml

A: 22ml; B: 22ml

2-6oC

ELISA control, positive, 1.8 ml/bottle

1

1

2

2-6oC

ELISA control, negative, 1.8 ml/bottle

1

1

2

2-6oC

PBST Washing buffer, powder

14.4 g

28.7 g

57.3 g

Room temperature

Tween-20, for PBST and sample buffer

4.0 g

7.0 g

14.0 g

Room temperature

SB1 General Sample buffer, powder*

5.8 g

11.6 g

23.2 g

Room temperature

SB4 grape sample buffer, powder*

24.7 g

49.4 g

98.7 g

Room temperature

SB5 blueberry sample buffer, powder*

8.6 g

17.3 g

34.5 g

Room temperature

Instruction

1

1

1

 

        Indirect ELISA, alkaline phosphatase conjugate, test instruction

Item

96 wells

192 wells

384 wells

Storage

SB3 Indirect sample buffer, 10x

25 ml

50 ml

100 ml

2-6oC

Detecting conjugate, alkaline phosphatase, Bottle A & Battle B, 1x

A: 6ml; B: 6ml

A: 12ml; B: 12ml

A: 23ml; B: 23ml

2-6oC

BluePhos substrate, Part A and Part B, 1x

A: 6ml; B: 6ml

A: 11ml; B: 11ml

A: 22ml; B: 22ml

2-6oC

ELISA control, positive, 1.8 ml/bottle

1

1

2

2-6oC

ELISA control, negative, 1.8 ml/bottle

1

1

2

2-6oC

96-well ELISA plates, empty

1

2

4

Room temperature

PBST Washing buffer, powder

14.4 g

28.7 g

57.3 g

Room temperature

Tween-20, for PBST and sample buffer

1.0 g

2.0 g

4.0 g

Room temperature

Instruction

1

1

1

 


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