Home    Products    Testing Services   Supplies   Contact Us  

                                                       PRODUCT ITEMS - ELISA Kit       

1.  Components of ELISA Kit

Coating Antibody Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate.
Detecting Conjugate Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate.
ELISA Plate Empty ELISA plate with high binding capacity.  The plate should be used for our ELISA Reagents to achieve a maximum test results.
Controls Positive control and negative control
Buffers Coating buffer, sample buffer, enzyme conjugate buffer, washing buffer, substrate buffer and PNP tablets.
Instruction Specific test instructions for each of the three different ELISA Formats.

2.  Contents of ELISA Reagents

        DAS ELISA, alkaline phosphatase conjugate

Item

500 wells

1000 wells

Storage

Coating antibody

0.25 ml

0.50 ml

4 oC

Detecting conjugate, alkaline phosphatase

0.25 ml

0.50 ml

4 oC

96-well ELISA plates

5

10

Room temperature

Positive control

1

2

4 oC

Negative control

1

2

4 oC

Coating buffer, 10x

6 ml

12 ml

Room temperature

Sample buffer powder

11.6 g

23.2 g

Room temperature

Enzyme conjugate buffer, 10x

6 ml

12 ml

Room temperature

PBST Washing buffer powder

28.7 g

57.23g

Room temperature

Tween-20 for sample/washing buffers

7.0 g

14.0 g

Room temperature

PNP substrate buffer, 5x

10 ml

20 ml

4 oC

PNP tablet, 5mg each

10 tablets

20 tablets

-10-20 oC

Instruction

1

1

 

        TAS ELISA, alkaline phosphatase conjugate

Item

500 wells

1000 wells

Storage

Coating antibody

0.25 ml

0.50 ml

4 oC

Detecting conjugate, alkaline phosphatase, 
(Bottles A and B)

0.25 ml (A)

0.25 ml (B)

0.50 ml (A)

0.50 ml (B)

4 oC

96-well ELISA plates

5

10

Room temperature

Positive control

1

2

4 oC

Negative control

1

2

4 oC

Coating buffer, 10x

6 ml

12 ml

Room temperature

Sample buffer powder

11.6 g

23.2 g

Room temperature

Enzyme conjugate buffer, 10x

6 ml

12 ml

Room temperature

PBST Washing buffer powder

28.7 g

57.23g

Room temperature

Tween-20 for sample/washing buffers

7.0 g

14.0 g

Room temperature

PNP substrate buffer, 5x

10 ml

20 ml

4 oC

PNP tablet, 5mg each

10 tablets

20 tablets

-10-20 oC

Instruction

1

1

 

        Indirect ELISA, alkaline phosphatase conjugate

Item

500 wells

1000 wells

Storage

Detecting conjugate, alkaline phosphatase, 
(Bottles A and B)

0.25 ml (A)

0.25 ml (B)

0.50 ml (A)

0.50 ml (B)

4 oC

96-well ELISA plates

5

10

Room temperature

Positive control

1

2

4 oC

Negative control

1

2

4 oC

Sample buffer powder

11.6 g

23.2 g

Room temperature

Enzyme conjugate buffer, 10x

6 ml

12 ml

Room temperature

PBST Washing buffer powder

28.7 g

57.23g

Room temperature

Tween-20 for washing buffers

7.0 g

14.0 g

Room temperature

PNP substrate buffer, 5x

10 ml

20 ml

4 oC

PNP tablet, 5mg each

10 tablets

20 tablets

-10-20 oC

Instruction

1

1

 


Home    Products    Testing Services   Supplies   Contact Us