Phakospsora pachyrhizi (Ppac) - TAS ELISA                      Home    Products    Testing Services   Supplies

Catalog #:  F241
Source Antibody: A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format: TAS ELISA  
                         
Reactivity:   This ELISA reacts specifically with Phakopsora pachyrhizi fungus. The assay detected the isolates of P. pachyrhizi in infected host plants such as soybean and lima bean.
Reaction of this test is very strong.  Optical Density at 405nm  is in a range of 1.000 - >3.000 depending on the fungal titers in the samples tested.

Sensitivity: This ELISA is very sensitive, and can detect the ASR fungus in pre‐symptomatic plant leaves.   The ASR pathogen can be detected at dilution of 1:21870 for the plant leaf samples prepared at 14 days after infection, and at dilution of 1:270 for the plant leaf samples prepared at 5 days after infection.

Specificity:  This test system is species‐specific for P. pachyrhizi.  This ELISA assay only reacts with the isolates of P. pachyrhizi species, and did not react with P. meibomiae. The antibodies used in this ELISA have high specificity to r369, and did not react with r107 protein.
This assay does not react with the other related rust fungi or any of the soybean pathogens tested, including  Uromyces appendisulatus, Puccinia punctiformis, Puccinia graminis, Puccinia graminis tritici, Rose rust, Fusarium graminearum, Downy mildew, Pseudomonas syringae , Pseudomonas syringae pv. phaseolicola,  Xanthomonas campestris pv. glycine, Ustilago tritici, Erysiphae polygono DC, Septoria lycopersici and Cercospora sojina.
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect the ASR fungus in pre-symptomatic plant leaves, and to detect the pathogen in many host plant tissues infected with P. pachyrhizi, such as leaves, stems, roots, seeds, and potato tubers, as well as in pure fungal cultures.

Immunodiagnostic assays targeted to urediniospore wall proteins of Asian soybean rust - USDA ARS Foreign Disease Weed Science Research Unit, Fort Detrick, MD

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To know more about the products:  Product Items
To learn how to perform the test:   Instructions for TAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Fungus

Name:  Phakopsora pachyrhizi
Acronym:  Ppac
Synonyms: 
Group/Genus: 
Transmission:  
Main host plants: 
Fungal Structures: 
Fungal Infection:  
Reference:

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