Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Peanut Stunt Virus -Western Strain (PSV-WS)-DAS ELISA Home Products Testing Services
Catalog #: V201
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all PSV-WS isolates tested. However, This test dose not reacted with the PSV typical strain. Reaction of the ELISA is strong. Optical Density at 405nm is in arrange of 1.000 - >2.600 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as peanut, bean, pea, tobacco, or other host plants. Background is very low on all of the negative control wells.
Application: The test can be used to detect PSV-WS in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V201-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V201-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V201-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V201-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V201-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V201-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V201-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V201-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V201-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V201-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V201-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V201-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V201-P1 | Positive control |
18 |
15 |
1 lb (0.5 kg) |
| V201-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Peanut Stunt Virus-Western Strain
Acronym: PSV-WS
Synonyms: robinia mosaic virus (Richter et al., 1979;
Schmelzer, 1971; Zheng and Kung, 1984), black locust true mosaic virus, clover
blotch virus (Richter et al., 1979).
Group/Genus: Cucumovirus
Vector: Transmitted by a vector; an insect; Aphis craccivora, A.
spiraecola and Myzus persicae, but not Aphis gossypii;
Aphididae. Transmitted in a non-persistent manner. .
Transmission: Virus transmitted by mechanical inoculation;
transmitted by seed (0.1% in Arachis hypogaea).
Main host plants: Robinia pseudoacacia, Trifolium repens, T.
pratense, T. vesiculosum, T. incarnatum, T. subterraneum, Medicago sativa,
Phaseolus spp., Arachis hypogaea, Vicia sp., Glycine
max, Pisum sativum, Vigna angularis, Coronilla varia, Tephrosia sp.,
Nicotiana tabacum, Apium graveolens, Lupinus luteus.
Virus Infection Symptoms: mosaic and leaf malformation, chlorotic
mottling, chlorotic local lesions and mottling, stunting, pod malformation, or
leaf chlorosis. Symptoms persist
Diagnostically susceptible host species and symptoms: Chenopodium
amaranticolor, C. quinoa - chlorotic local lesions; systemic spotting;
Spinacia oleracea, Tetragonia tetragonioides - systemic mottling; Glycine
max - systemic chlorotic mottling; Phaseolus vulgaris - chlorotic or
necrotic local lesions, systemic chlorotic mottling or mosaic; P. vulgaris
cv. Bountiful - elongated mis-shapen trifoliate leaves; Pisum sativum -
systemic chlorotic mottling, stunting; Vigna unguiculata - chlorotic
local lesions; systemic vein clearing and severe epinasty; Datura stramonium
- systemic chlorotic mottle with islands of green tissue; Nicotiana tabacum
- light green and yellow rings, then systemic chlorotic areas; Tetragonia
tetragonioides - systemic mottle.
References:
1. Karasawa, A., Nakaho, K., Kakutani, T., Minobe, Y. and Ehara, Y.
(1991). Virology 185: 464.
2. Mink, G.I. (1972). CMI/AAB Descr. Pl. Viruses No. 92, 4
pp.
3. Xu, Z., Barnett, O.W. and Gibson, P.B. (1986). Phytopathology
76: 390