Plum Pox Virus (PPV) - TAS ELISA                                             Home    Products    Testing Services   Supplies

Catalog #:  V080
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system detects all isolates or strains of virus including D-strain and M-strain. Reaction of the ELISA is relatively strong.  Optical Density at 405nm  is in a range of 1.000 - 2.800 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant leaf tissues diluted  at 1:810 - 1:2430.

Specificity:  This test does show some cross-reaction with some of other potyviruses. There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect PPV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V080-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V080-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V080-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V080-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V080-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V080-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V080-C1

Coating Antibody

500

115

2 lb (1 kg)

V080-C2

Coating Antibody

1000

205

2 lb (1 kg)

V080-C3

Coating Antibody

5000

725

2 lb (1 kg)

V080-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V080-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V080-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)
VS080-LF1 ASPOT√CHECK LF™, lateral flow device (Dip Strip), complete kit for real-time quick assay 10 125 2 lb (1 kg)
VS080-LF2 ASPOT√CHECK LF™, lateral flow device (Dip Strip), complete kit for real-time quick assay 100 1240 13 lb (6 kg)
V080-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V080-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Plum Pox Virus
Acronym:  PPV
Synonyms:  Annulus pruni, Prunus virus 7,  sharka virus.
Group/Genus:  Potyviruses

Vector:  Aphids, in non-persistent manner.
Transmission:  mechanical
Main host plants:  Plum, peach, cherry, apricot, nectarine, almond, European plum, Janpanese plum

Viral Particle:  Filamentous particle, containing RNA genome.
Virus Infection: 

1.  CMI/AAB Descriptions of Plant Viruses, No.70.
2.  Adams, A. N. 1995. In Ogawa, J.M., ed. Compendium of Stone Fruit Diseases, APS Press, St. Paul. p. 69. (ISBN 0-89054-174-4)
3.  Candresse, T., Cambra, M., Dallot, S., Lanneau, M., Asensio, M., Gorris, M.T., Revers, F., Macquaire, A., Olmos, A., Boscia, D., Quiot, J.B., dunez, J. (1998) Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of Plum Pox Potyvirus. Phytopathology 88(3), 198-204.


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