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Conjugate: All the antibodies are conjugated with enzyme alkaline phosphatase. Conjugates with horseradish peroxidase are available by the customer request. ELISA format: Standard ELISA formats of double antibody sandwich (DAS), triple antibody sandwich (TAS) and indirect ELISA (Indirect) are used in the products. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ PRODUCT ITEMS: ELISA Reagents: DAS ELISA Coating PAb or MAb plus specific antibody enzyme conjugate. TAS ELISA Coating PAb or MAb plus detecting MAb or PAb and anti-species antibody enzyme conjugate. Indirect ELISA Detecting MAb or PAb and anti-species antibody enzyme conjugate. Coating Antibody: Specific capture PAb or MAb for coating ELISA plate. Detecting Conjugate: DAS ELISA Specific antibody enzyme conjugate. TAS ELISA Detecting MAb or PAb and anti-species antibody enzyme conjugate. Indirect ELISA Detecting MAb or PAb and anti-species antibody enzyme conjugate. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ TO PLACE YOUR ORDER OR MAKE ENQUIRIES Write to: ADI, Inc., 53490 Old Farm Road Elkhart, IN 46514 USA Tel: (219) 674-8097 Fax:: (219) 674-8097 Web Site: www.adixia.com E-mail: acjqxia@juno.com ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ PRICES AND TERMS Payment is due in $US within 30 days of receipt of invoice. Shipping cost is added in invoice. Payment in advance may be required. In some cases, value added tax (VAT) will be added to the invoice price of goods in shipping. Prices may be changed without notice. For price assurance, contact us. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ PLACING AN ORDER 1. Please include: Product catalog number and description. Contact name and title. Delivery address and billing address. Telephone number and/or fax number/e-mail address. Call for placing orders. Also, fax, e-mail or mail or your orders. Delivery will be made in 3-5 working days. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ PAYMENT METHODS Payment may be made by US checks or by direct transfer of all charges paid. Other payment options are available on request. We will accept payment by major credit cards soon. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Primary Business Address 53490 Old Farm road Elkhart, IN 46514 USA ADI, Inc. f& 53490 Old Farm Road, Elkhart, IN 46514, USA f& Phone: 219-674-8097 f& Web: www.adixia.com ADI, Inc. ADI, Inc. f& 53490 Old Farm Road, Elkhart, IN 46514, USA f& Phone: 219-674-8097 f& Web: www.adixia.com Welcome to ADI s catalog 2000! Advanced Diagnostics International, Incorporated (ADI, Inc.) is a newly established agri-diagnostic company. ADI was founded because we believe today s most advanced agricultural diagnostic products could and should be offered at affordable prices to worldwide customers. ADI is dedicated to the double pillars of high quality and low prices. Our products are developed in collaboration with scientists in the United states and many other countries. All of the products are produced by advanced manufacturing techniques and passed strict quality control procedures. Currently, we provide ELISA Reagents for the detection of more than 90 plant viruses. Many more diagnostic kits for plant pathogens and agricultural products are in development. If you are interested in these products which are not in this catalog, please let us know. Our researchers in R&D will be happy to meet your needs. Because of our high product quality and technical support, ADI guarantees 100% customer satisfaction. We would like to offer our new customers some of our products for absolutely free! We will send you a free sample of 250 test wells of any one of the ELISA Reagents you select from this catalog. Therefore, you can try it and see the ADI quality for yourself. Also, we encourage you to compare our prices with those of other companies before making your purchasing decision. Thank you for your interest in ADI, Inc. Sincerely, the team at ADI, Inc. ADI, Inc. 53490 Old Farm Road Elkhart, IN 46514, USA Tel: (219) 6748097 Email: acjqxia@juno.com Welcome to ADI s catalog 2000! Advanced Diagnostics International, Incorporated (ADI, Inc.) is a newly established agri-diagnostic company. ADI was founded because we believe today s most advanced agricultural diagnostic products could and should be offered at affordable prices to worldwide customers. ADI is dedicated to the double pillars of high quality and low prices. Our products are developed in collaboration with scientists in the United states and many other countries. All of the products are produced by advanced manufacturing techniques and passed strict quality control procedures. Currently, we provide ELISA Reagents for the detection of more than 90 plant viruses. Many more diagnostic kits for plant pathogens and agricultural products are in development. If you are interested in these products which are not in this catalog, please let us know. Our researchers in R&D will be happy to meet your needs. Because of our high product quality and technical support, ADI guarantees 100% customer satisfaction. We would like to offer our new customers some of our products for absolutely free! We will send you a free sample of 250 test wells of any one of the ELISA Reagents you select from this catalog. Therefore, you can try it and see the ADI quality for yourself. Also, we encourage you to compare our prices with those of other companies before making your purchasing decision. Thank you for your interest in ADI, Inc. Sincerely, The team at ADI, Inc. ADI, Inc. 53490 Old Farm Road Elkhart, IN 46514, USA Tel: (219) 6748097 Web: www.adixia.com Email: acjqxia@juno.com Index To The Tests Available Index To The Tests Available Name Acronym Page Name Acronym Page African cassava mosaic virus ACMV 1 Pea mosaic virus PMV 7 Alfalfa mosaic virus AMV Pea seedborn mosaic virus PSbMV 8 Alstroemeria mosaic virus AIMV 1 Peach rosette mosaic virus PRMV 8 Andean potato latent virus APLV 1 Pelargonium flower break virus PFBV 8 Andean potato mottle virus APMoV 1 Pelargonium line pattern virus PLPV 8 Apple mosaic virus ApMV 1 Pelargonium zonate spot virus PZSV 8 Arabis mosaic virus ArMV 1 Pepper mild mottle virus PMMoV 8 Barley stripe mosaic virus BSMV 1 Pepper mottle virus PepMoV 8 Barley yellow dwarf viru mav BYDV-mav 2 Plum pox virus PPV 9 Barley yellow dwarf virus -pav BYDV-pav 2 Poinsettia mosaic virus PnMV 9 Barley yellow dwarf virus rpv BYDV-rpv 2 Potato black ringspot virus PBRSV 9 Bean common mosaic virus BCMV 2 Potato leafroll virus PLRV 9 Bean leaf roll virus BLRV 2 Potato virus A PVA 9 Bean pod mottle virus BPMV 2 Potato virus M PVM Bean yellow mosaic virus BYMV 2 Potato virus S PVS 9 Beet necrotic yellow vein virus BNYVV Potato virus V PVV 9 Beet western yellows virus BWYV 3 Potato virus X PVX 10 Blackeye cowpea mosaic virus BICMV 3 Potato virus Y PVY 10 Blackgram mottle virus BMoV 3 Potato virus Y - n strain PVY-n 10 Blueberry scorch virus BlSV 3 Potato yellow dwarf virus PYDV 10 Blueberry necrotic shock virus BlShV 3 Potyvirus group POTY 10 Broad bean wilt virus BBWV 3 Prunus necrotic ringspot virus PNRSV 10 Carnation etched ring virus CERV 3 Raspberry bushy dwarf virus RBDV 10 Carnation mottle virus CarMV 4 Raspberry ringspot virus RpRSV 11 Carnation ringspot virus CRSV 4 Red clover vein mosaic virus RCVMV 11 Cauliflower mosaic virus CaMV 4 Ribgrass mosaic virus RMV 11 Cherry leaf roll virus CLRV 4 Shallot virus X ShVX 11 Chrysanthemum virus B CVB 4 Southern bean mosaic virus SBMV 11 Citrus tristeza virus CTV 4 Soybean mosaic virus SMV 11 Clover yellow vein virus ClYVV 4 Squash mosaic virus SqMV 11 Cowpea chlorotic mottle virus CCMV 5 Strawberry latent ringspot virus SLRSV 12 Cowpea mosaic virus CPMV 5 Strawberry mild yellow edge virus SMYEaV 12 Cowpea severe mosaic virus CPSMV 5 Tobacco mosaic virus TMV 12 Cucumber green mottle mosaic virus CGMMV 5 Tobacco necrosis virus TNV 12 Cucumber mosaic virus CMV 5 Tobacco rattle virus TRV 12 Cymbidium mosaic virus CymMV 5 Tobacco ringspot virus TRSV 12 Cymbidium ringspot virus CymRSV 6 Tobacco streak virus TSV 12 Eggplant mosaic virus EMV 6 Tobacco vein mottling virus TVMV Garlic common latent viru GCLV 6 Tomato aspermy virus TAV 13 Grapevine fanleaf virus GFLC 6 Tomato black ring virus - g strain TBRV- g 13 Hop american latent virus AHLV 6 Tomato bushy stunt virus TBSV 13 Impatiens necrotic spot virus INSV 6 Tomato mosaic virus ToMV 13 Iris fulva mosaic virus IFMV 6 Tomato ringspot virus ToRSV 13 Leek yellow stripe virus LYSV 6 Tomato spotted wilt virus TSWV 13 Lettuce mosaic virus LMV 7 Turnip mosaic virus TuMV 14 Melon necrotic spot virus MNSV 7 Watermelon mosaic virus 2 WMV-2 14 Odontoglossum ringspot virus ORSV 7 Wheat spindle streak mosaic virus WSSMV 14 Onion yellow dwarf virus OYDV 7 wheat streak mosaic virus WSMV 14 Papaya ringspot virus PRSV 7 White lupine mosaic virus WLMV 14 Pea enation mosaic virus PEMV 7 Zucchini yellow mosaic virus ZYMV 14 Catalog Description Test Price Catalog Description Test Price Number Well $US Number Well $US African Cassava Mosaic Virus (ACMV) PAb+PAb In DAS ELISA V001-R1 ELISA Reagents 500 150 V001-R2 ELISA Reagents 1000 250 V001-R3 ELISA Reagents 5000 1000 V001-C1 Coating Antibody 500 70 V001-C2 Coating Antibody 1000 110 V001-C3 Coating Antibody 5000 420 V001-D1 Detecting conjugate 500 90 V001-D2 Detecting conjugate 1000 150 V001-D3 Detecting conjugate 5000 620 V001-P1 Positive control 15 9 V001-N1 Negative control 15 9 Alfalfa Mosaic Virus (AMV) PAb+MAb In TAS ELISA V099-R1 ELISA Reagents 500 140 V099-R2 ELISA Reagents 1000 220 V099-R3 ELISA Reagents 5000 900 V099-C1 Coating Antibody 500 65 V099-C2 Coating Antibody 1000 95 V099-C3 Coating Antibody 5000 380 V099-D1 Detecting conjugate 500 85 V099-D2 Detecting conjugate 1000 135 V099-D3 Detecting conjugate 5000 540 V099-P1 Positive control 15 9 V099-N1 Negative control 15 9 Alstroemeria Mosaic Virus (AIMV) PAb+PAb In DAS ELISA V002-R1 ELISA Reagents 500 150 V002-R2 ELISA Reagents 1000 250 V002-R3 ELISA Reagents 5000 1000 V002-C1 Coating Antibody 500 70 V002-C2 Coating Antibody 1000 110 V002-C3 Coating Antibody 5000 420 V002-D1 Detecting conjugate 500 90 V002-D2 Detecting conjugate 1000 150 V002-D3 Detecting conjugate 5000 620 V002-N1 Negative control 15 9 V056-R1 ELISA Reagents 500 100 V056-R2 ELISA Reagents 1000 160 Andean Potato Latent Virus (APLV) PAb+PAb In DAS ELISA Page # Secondary Heading The purpose of a catalog is to sell products or services to a targeted audience, or to advertise upcoming classes or events. Catalogs can be a great way to market your products or services, and also build your organization s identity. Secondary Heading First, determine the audience of the catalog. This could be anyone who might benefit from the products or services it contains. Next, establish how much time and money you can spend on your catalog. These factors will help determine the length of the catalog and how frequently you publish it. It s recommended that you publish your catalog at least quarterly so that it s considered a consistent source of information. Your customers or employees will look forward to its arrival. Also consider how you want to print your catalog. You can print it on a desktop printer, at a copy shop, or at a commercial printing service. In addition to your budget, the complexity of the publication including whether you print it as a black and white or a color publication will help determine the best method for printing your publication. Before you print your catalog, consider how you will bind your pages. The number of pages, how the reader will use it, and whether you mail it will help you determine the type of binding. For example, if you have only a few pages and your catalog is meant to be held, you might consider folding the pages and stapling the spine. Larger catalogs that are meant to be folded flat work best with a plastic binding with punched holes, while larger publications meant to be held like a book are best served by gluing the pages, or  perfect binding. ACD, Inc.%Web: www.acdiainc.com%E-mail: infor@acdiainc.com%Ph: (479) 595-0320%Fax: (479) 251-1791 Page # ELISA Reagents DAS ELISA, alkaline phosphatase conjugate Content List Safety and Storage Always wash hands thoroughly after using this product. Prevent direct skin and eye contact with, or ingestion of, product components. Obtain medical attention in case of accidental ingestion of reagent components. All reagent components should be stored at the recommended temperature to assure their full shelf life. Do not store prepared working solution from day to day. Please contact ACD, Inc. if you have any questions about safety and storage of this product. Preparing For The Test Check all the components in the package of ELISA Reagents. Prepare all buffer solutions according to the attached buffer formulations. Make sure all laboratory equipments and facilities required for the test are ready. Prepare a humid box for incubation steps. Make a copy of the attached recording sheet and create a loading diagram by recording the locations of your samples, controls, and other reagents needed. Coating Plate With Antibody Lay out all items that will be required for the plate coating step before beginning. Prepare coating antibody in a container made of glass, polyethylene or any material that does not readily bind coating antibody. Coat the plate immediately after preparing the coating antibody. Some coating antibody can be lost if too much time elapses between diluting the coating antibody and coating the plate. The volume of coating buffer required depends on the number of test wells used; 100 l is needed per test well. One way to estimate the volume needed is to prepare 1 ml of coating buffer for each 8well strip used, or 10 ml for each 96well plate. Dilute the concentrated coating antibody into coating buffer at the dilution given on the label. Mix well. Always prepare coating antibody immediately before use. Pipette 100 l of coating antibody into each well. Incubate the plate in a humid box for overnight in the refrigerator (4 C) or 4 hours at room temperature (21-24 C). Preparing Samples Select symptomatic and/or infective tissues for the test. Leaf tissue is often used in ELISA testing. Plant tissues such as stem, sprout, seed, tuber, root and others can also be used. We suggest that each test well be used for only one sample. In some cases, composites of up to ten leaves per test well can be used to make testing more economical. However, too many plant samples per well can reduce the sensitivity of the test. ACD s SB1 buffer can be used as extraction buffer for most of the plant samples. However, other buffers are also recommended for some plant species. Grind sample with a mortar and pestle, or other grinding device. If you are using a mortar and pestle, wash and rinse it thoroughly between samples. If you extract plant sap, dilute the sap into sample extraction buffer at a ratio of 1:10 (sap volume: buffer volume). Or you can grind plant tissue in extraction buffer at a 1:10 ratio (tissue weight: Extraction Buffer volume). If you have any questions about sampling, sample preparation, or the appropriate extraction buffer for your samples, please contact ACD, Inc. Plate Washing Wash the plate when the incubation is complete. Use a quick flipping motion to empty the wells into a sink or waste container. Wash the plate by filling the wells with PBST, then quickly emptying them again. Repeat 4 to 6 times. To remove drops of PBST from the wells after washing, hold the frame upside down and tap firmly on a folded paper towel. Sample Dispensing and Incubation About 100 l of diluted sample extract is needed per test well. Always have an additional amount to assure easy dispensing. A convenient way to prepare this diluted sample is to measure 100 l of undiluted sap into a small test tube, then add 1 ml of extraction buffer. Following your loading diagram on your recording sheet, dispense 100 l of prepared sample into sample wells. Dispense 100 l of positive control into positive control wells, and dispense 100 l of negative control or extraction buffer into negative control wells. Put the plate inside the humid box and incubate for 2.5 hours at room temperature (21-24 C) or overnight in the refrigerator (4 C). Wash the plate by filling the wells with PBST, then quickly emptying them again. Repeat 4 to 6 times. Page # Lot Number Item 500 wells 1000 wells 5000 wells Storage Coating antibody 0.25 ml 0.50 ml 2x1.25 ml 4 oC Detecting conjugate, alkaline phosphatase 0.25 ml 0.50 ml 2x1.25 ml 4 oC 96well ELISA plates 5 10 50 Room temperature Instruction 1 1 1 . Wash the plate by filling the wells with PBST, then quickly emptying them again. Repeat 4 to 6 times. To remove drops of PBST from the wells after washing, hold the frame upside down and tap firmly on a folded paper towel. Sample Dispensing and Incubation About 100 l of diluted sample extract is needed per test well. Always have an additional amount to assure easy dispensing. A convenient way to prepare this diluted sample is to measure 100 l of undiluted sap into a small test tube, then add 1 ml of extraction buffer. Following your loading diagram on your recording sheet, dispense 100 l of prepared sample into sample wells. Dispense 100 l of positive control into positive control wells, and dispense 100 l of negative control or extraction buffer into negative control wells. Put the plate inside the humid box and incubate for 2.5 hours at room temperature (21-24 C) or overnight in the refrigerator (4 C). Preparing Enzyme Conjugate Always make enzyme conjugate solution within 10 minutes before use. Prepare the enzyme conjugate, using ACD s ECB1 buffer and a cleaning container. The volume of ECB1 buffer required depends on the number of test wells used; 100 l are needed per test well. To estimate the volume needed, prepare 1 ml for each 8well strip used, or 10 ml for each 96well plate. The volume of enzyme conjugate required for each test is calculated based on the volume of ECB1 buffer used and on the dilutions given on the bottles. Use a new, sterile pipette tip and change the tip for each pipetting to prevent contamination. First dispense appropriate volume of ECB1 buffer into a cleaning container, then add enzyme conjugate according to the dilution given on the label. Mix the conjugate solution thoroughly. It is important to mix the enzyme conjugate well for a consistent test result. Prepare enzyme conjugate just before use. Keep the prepared enzyme conjugate at a safe place and use it after washing the plate. Washing Plate Wash the plate when the incubation is complete. Use a quick flipping motion to empty the wells into a sink or waste container without mixing the contents. Wash the plate by filling the wells with PBST, then quickly emptying them again. Repeat 6 to 8 times. To remove drops of PBST from the wells after washing, hold the frame upside down and tap firmly on a folded paper towel. Enzyme Conjugate Incubation Dispense 100 l of prepared enzyme conjugate per well for all test wells. Incubate the plate in the humid box for 2.5 hours at room temperature (21-24 C). Preparing Substrate Solution Concentration of PNP in substrate is 1 mg/ml. Each PNP tablet will make 5 ml of PNP solution, which is enough for 48 test wells or five 8well strips. Do not touch the PNP tablets or expose the PNP solution to strong light. Light or contamination could cause background color in negative wells. Prepare PNP substrate about 10-15 minutes before the end of the above incubation step. Measure 5 ml of PNP buffer for each tablet, then add the PNP tablets to the buffer. Mix by vortexing or stirring to let the PNP tablet fully dissolve in the buffer. Washing plate Wash the plate 6 to 8 times with PBST as instructed above. Incubation With Substrate Dispense 100 l of PNP substrate solution per well. Incubate the plate for 30 to 60 minutes in a humid box at room temperature (21-24 C). To stop reaction, add 50 l of 3M sodium hydroxide to each well. This step is optional. The plate can be interpreted visually or with a plate reader without adding the stop solution. Evaluating Results Test results can be examined by eye, or measured on a plate reader at 405 nm. Development of yellow color in test wells indicate positive results. Wells in which there is no significant color development indicate negative results. Test results are valid only if positive control wells give a positive result and negative control wells remain clear. Results may be interpreted after more than 60 minutes of incubation as long as negative control wells remain virtually clear. ACD, Inc.%Web: www.acdiainc.com%E-mail: infor@acdiainc.com%Ph: (479) 595-0320%Fax: (479) 251-1791 Page # Buffer Formulations Coating Buffer Sodium carbonate (anhydrous) 1.60 g Sodium bicarbonate 2.92 g Sodium azide 0.2 g Dissolve in distilled water and make to 1000 ml. Adjust pH to 9.6. Store at 4 C. PBST Buffer Sodium phosphate, dibasic, (anhydrous) 1.15 g Potassium phosphate, monobasic (anhydrous) 0.2 g Sodium chloride 8.0 g Potassium chloride 0.2 g Tween20 0.5 g Dissolve in distilled water and make to 1000 ml. Adjust pH to 7.3. SB1 Buffer Powdered egg (chicken) albumin, Grade II 2.0 g Polyvinylpyrrolidone (PVP) MW, 2440,000 10.0 g Sodium sulfite (anhydrous) 1.3 g Sodium azide 0.2 g Tween20 10.0 g Dissolve in 1000 ml of 1X PBST. Adjust pH to 7.3. Store at 4 C. ECB1 Buffer Bovine serum albumin (BSA) 2.0 g Polyvinylpyrrolidone (PVP) MW 2440,000 10.0 g Sodium azide 0.2 g Dissolve in 1000 ml of 1X PBST. Adjust pH to 7.3. Store at 4 C. PNP Buffer Diethanolamine 97.0 ml Magnesium chloride 0.1 g Sodium azide 0.2 g Dissolve in 800 ml distilled water. Adjust pH to 9.8 with hydrochloric acid. Adjust final volume to 1000 ml with distilled water. Store at 4 C. ACD, Inc.%Web: www.acdiainc.com%E-mail: infor@acdiainc.com%Ph: (479) 595-0320%Fax: (479) 251-1791 Page # RECORDING SHEET FOR ELISA TEST: DATE: BY: TIMING: Coating Sample EC Substrate: KEY POINTS: Coating Antibody: ul, Coating Buffer: ml, Enzyme Conjugate: ul, ECB1 ml PNP Substrate: ml RESULTS/CONCLUSIONS: 1. 2. 3. 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