Clavibacter michiganensis pv. michiganensis (Cmm) - DAS ELISA             Home    Products    Testing Services   Supplies

Catalog #:  B103
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all Cmm isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm  is in a range of 1.100 - >3.000 depending  on the bacterial titers in the samples to be tested.

Sensitivity:  Sensitivity of the ELISA is high.  The bacterium can be consistently detected in infected plant leaf tissues diluted at 1:810 - 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, E. chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  This test shows cross-reaction with Clavibacter michiganensis subsp. insidiosus and C. m. subsp. nebraskensis. 
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Cmm in infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

B103-R1

ELISA Reagents, Alkaline Phosphatase

500

220

2 lb (1 kg)

B103-R2

ELISA Reagents, Alkaline Phosphatase

1000

385

3 lb (1.5 kg)

B103-R3

ELISA Reagents, Alkaline Phosphatase

5000

1515

12 lb (6 kg)
B103-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
B103-K1 ELISA Kit, Alkaline Phosphatase

500

340

2 lb (1 kg)
B103-K2 ELISA Kit, Alkaline Phosphatase

1000

530

3 lb (1.5 kg)

B103-C1

Coating Antibody

500

110

2 lb (1 kg)

B103-C2

Coating Antibody

1000

195

2 lb (1 kg)

B103-C3

Coating Antibody

5000

690

2 lb (1 kg)

B103-D1

Detecting conjugate, Alkaline Phosphatase

500

135

2 lb (1 kg)

B103-D2

Detecting conjugate, Alkaline Phosphatase

1000

215

2 lb (1 kg)

B103-D3

Detecting conjugate, Alkaline Phosphatase

5000

860

2 lb (1 kg)

B103-P1

Positive control

18

18

1 lb (0.5 kg)

B103-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Bacterium

Name:  Clavibacter michiganensis pv. michiganensis
Acronym:  Cmm
Synonyms: 
Group/Genus:  Clavibacter

Host plants and Diseases:  Bacterial canker of tomato, 
Symptoms:  Plant wilting, round spot on fruits, stems and leaves.

Bacterial Transmission:  Seeds, infected plant residues, infected stems in soil, agricultural equipments.
Bacterial Infection:  Through wounds and natural openings such as stomata and trichomes.

Reference:  


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