Catalog #: B103
Reactivity: This test system reacted with all Cmm isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in a range of 1.100 - >3.000 depending on the bacterial titers in the samples to be tested.
Sensitivity: Sensitivity of the ELISA is high. The bacterium can be consistently detected in infected plant leaf tissues diluted at 1:810 - 1:2430.
Specificity: This test system reacts
negatively with Acidovorax avenae subsp. citrulli, Clavibacter
flaccumfaciens, C. michiganensis subsp. sepedonicus, C. m. subsp. tessellarius,
Erwinia carotovora subsp. atroseptica, E.
c. subsp. carotovora, E. chrysanthemi,
E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv.
syringae, Ralstonia solanacearum, Xanthomonas campestris pv.
armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c.
pv. diffenbachiae, X. c. pv. pelargonii, X.
c. pv. phaseoli, and X. c. pv. vesicatoria. This test shows cross-reaction with Clavibacter
michiganensis subsp. insidiosus and C. m. subsp. nebraskensis.
Application: The test can be used to detect Cmm in infected host plants as well as in pure bacterial cultures.
It is easy and convenient To Place
Information About the Bacterium
Name: Clavibacter michiganensis pv.
Host plants and Diseases: Bacterial canker of tomato,
Bacterial Transmission: Seeds, infected plant residues,
infected stems in soil, agricultural equipments.
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