Cherry Rasp Leaf Virus (CRLV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V145
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CRLV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405_nm  is in arrange of 0.700 - >2.100 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:270 - 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as cherry or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect CRLV in infected cherry and other infected woody plants as well as herbaceous host plants.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Cherry Rasp Leaf Virus
Acronym:  CRLV
Synonyms:  flat apple virus
Group/Genus:  Nepovirus

Vector:  Transmitted by a vector; a nematode; Xiphinema americana (Nyland et al., 1969; Hansen et al., 1974); Dorylamidae.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (10-20%).
Main natural host plants: Prunus avium, P. mahaleb, Prunus persica, Malus sylvestris, Balsamorhiza sagittata, Taraxacum officinale, Plantago major.

Virus Infection:  affected cherry branches are very frost sensitive (Bodine et al., 1951; Hansen et al., 1974; Wagnon et al., 1968) enations, stunting. Infected other host plant show nations, stunting, decline, flat fruit or symptomless.
Diagnostically susceptible host species and symptoms: Cucumis sativus - chlorotic local lesions; systemic mottle; Cyamopsis tetragonoloba - necrotic local lesions; not systemic; Chenopodium quinoa - mottle and vein clearing; Chenopodium amaranticolor - systemic mottle; Vigna unguiculata, Physalis floridana, Sesbania exaltata - local lesions but unreliable..

Geographical distribution: Spreads in the North American region and the Pacific region; Canada, New Zealand, South Africa, and the USA. Found, but with no evidence of spread, in the U.K. (Jones et al., 1985), China, Australia and New Zealand and possibly South Africa. Prunus avium raspleaf symptoms have been found in many countries associated with one or another of seven viruses (Hansen et al., 1974).

References:
1.  Jones, A.T., Mayo, M.A. and Henderson, S.J. (1985). Ann. appl. Biol. 106: 101.
2.  Stace-Smith, R. and Hansen, A.J. (1976). CMI/AAB Descr. Pl. Viruses No. 159, 4 pp.

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