Beet Yellows Closterovirus (BYV) - DAS ELISA                        Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V254
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:                                   

Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.700 - 1.900 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is very low on the negative control wells.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V254-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V254-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V254-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V254-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V254-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V254-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V254-C1

Coating Antibody

500

125

2 lb (1 kg)

V254-C2

Coating Antibody

1000

235

2 lb (1 kg)

V254-C3

Coating Antibody

5000

805

2 lb (1 kg)

V254-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V254-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V254-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V254-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V254-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

*IDENTIKIT™ offers the quantitative or semi-quantitative detection of a chosen pathogen.

It is easy and convenient:
To Place An Order 

To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Beet Yellows Closterovirus
Acronym:  BYV
Genus:  Closterovirus
Synonyms: sugarbeet yellows closterovirus.

Strains: strains of differing pathogenicity are known to exist.       
Transmission: Transmitted by a vector; an insect; Myzus persicae, Aphis fabae; Aphididae. Not transmitted by Rhopalosiphum padi, Macrosiphum (Sitobion) avenae. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; transmitted by mechanical inoculation (with difficulty); transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical Distribution:   Probably distributed worldwide (wherever Beta vulgaris is grown). Spreads in China.
Diagnostically Susceptible Species and Symptoms:  Tetragonia tetragonioides - stunting, vein clearing, yellowing. Montia perfoliata - necrotic spots, reddening and yellowing of older leaves.

References:
Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Lunina, N.A., Koonin, E.V. and Dolja, V.V. (1991). J. gen. Virol. 72: 15.
Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991). J. mol. Biol. 217: 603.


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