Blueberry Leaf Mottle Virus (BLMV) - DAS ELISA                                        Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V004BLMV
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:                                   

Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 1.200 - 3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system has no cross reaction with healthy plant tissues tested.Background is low on all of  the negative control wells.

Application:  The test can be used to detect BLMV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V004-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V004-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V004-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V004-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V004-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V004-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V004-C1

Coating Antibody

500

125

2 lb (1 kg)

V004-C2

Coating Antibody

1000

235

2 lb (1 kg)

V004-C3

Coating Antibody

5000

805

2 lb (1 kg)

V004-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V004-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V004-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V004-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V004-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase
500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase   1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000 
400
6 lb (3.0 kg)


It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Blueberry Leaf Mottle Nepovirus
Acronym:  BLMV
Synonyms: 
Genus:  Nepovirus

Strains: blueberry leaf mottle virus - M1 and blueberry leaf mottle virus - NY.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; transmitted by pollen to the pollinated plant (by honeybees).

Geographical Distribution: Spreads in the USA (Michigan). Found, but with no evidence of spread, in New York, USA (grapevine) and in Pleven, Bulgaria.
Diagnostically Susceptible Species and Symptoms:  Chenopodium quinoa, C. album - chlorotic local lesions, systemic mottle and tip necrosis. C. amaranticolor - systemic mottle. Nicotiana clevelandii - systemic necrosis

References:
Childress, A.M. and Ramsdell, D.C. (1986a). Acta Hort. 186: 87.
Ramsdell, D.C. (1987). In: Virus Diseases of Small Fruits; ed. R.H. Converse. U.S. Dep. Agric. Hdbk No. 631, p. 112.


 


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