Beet Curly Top Virus (BCTV) - TAS ELISA               Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V005
Source Antibody:  A rabbit anti-BCTV polyclonal antibody (UID16) and guinea pig anti-BCTV polyclonal antibody (GP-1) for detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system reacted with all BCTV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in a range of 0.900 - >2.500 depending  on the virus titer in the samples tested. This test reacts with three Curtovirus species beet severe curly top virus (BSCTV), beet mild curly top virus (BMCTV), and beet curly top virus (BCTV).

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant leaf tissues diluted  at
1:810-1: 2430

Specificity:  There is no cross reaction with healthy beet plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect BCTV , BMCTV and BSCTV in infected beet plant tissues including roots, stems and leaves.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V005-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V005-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V005-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V005-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V005-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V005-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V005-C1

Coating Antibody

500

120

2 lb (1 kg)

V005-C2

Coating Antibody

1000

215

2 lb (1 kg)

V005-C3

Coating Antibody

5000

750

2 lb (1 kg)

V005-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V005-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V005-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)

V005-P1

Positive control

18

20

1 lb (0.5 kg)

V005-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

It is asy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Beet Curly Top Virus
Acronym:  BCTV
Family: Geminiviridae 
Group/Genus: Curtovirus
 
Curly top is caused by any of three Curtovirus species Beet severe curly top virus (BSCTV), Beet mild curly top virus (BMCTV), and Beet curly top virus (BCTV).

Transmission:  Transmitted by a vector; an insect; Circulifer tenellus in N. America, C. tenellus, C. opacipennis in Mediterranean Basin; Cicadellidae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; not transmitted by mechanical inoculation (unless special procedures used); transmitted by grafting (by dodder from plants that are not hosts of the vector); not transmitted by seed.

Main host plants:
 Beta vulgaris - vein-clearing and upward and inward rolling of leaves. Vein swelling and galling to give rough abaxial leaf surface. Phloem exudate on petioles and large leaf veins, phloem necrosis in roots. Cucumis sativus - seedlings killed. Older plants stunted and malformed, leaves rolled upward. Phaseolus vulgaris cv. Bountiful - first trifoliate leaf epinastic. Buds of survivors proliferate, leaflets brittle, curled and crinkled.

Geographical distribution: Spreads in the African region, the Eurasian region, the Mediterranean region, the North American region, and the South and Central American region (in the arid and semi-arid regions of the west); Argentina, Bolivia, Brazil, Canada, Costa Rica, Cyprus, Egypt, India, Iran, Italy, Mexico, Puerto Rico, Spain, Turkey, the USA, and Uruguay.

Reference:
1. Bennett, Monogr. Am. Phytopath. Soc. No. 7, 81 pp., 1971.
2. Egbert, Egbert & Mumford, Abs. Ann. Meet. Am. Soc. Microbiol. 1976, p. 258, 1976.
3. Esau, J. Ultrastruct. Res. 61: 78, 1977.
4. Mumford & Thornley, Phytopathology News 12:192, 1978.
5. Thomas, Phytopathology 67: 903, 1977.


Home    Products    Testing Services   Supplies   Contact Us