Broad Bean True Mosaic Virus (BBTMV) - DAS ELISA                 Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V270
Source Antibody:  A rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BBTMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.400 - >1.700 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:30 -1:90.

Specificity:  There is no cross reaction with healthy plant  tissues such as apple or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect BBTMV in infected herbaceous host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V270-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V270-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V270-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V270-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V270-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V270-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V270-C1

Coating Antibody

500

125

2 lb (1 kg)

V270-C2

Coating Antibody

1000

235

2 lb (1 kg)

V270-C3

Coating Antibody

5000

805

2 lb (1 kg)

V270-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V270-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V270-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V270-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V270-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase
1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Broad Bean True Mosaic Virus
Acronym:  BBTMV
Synonyms:  Vicia virus 1, Echtes Ackerbohnenmosaikvirus.
Group/Genus:  Comovirus

Vector: Transmitted by a vector; an insect; Apion vorax, Sitonia spp. and other weevils; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed.
Main natural host plants: Pisum sativum, Vicia faba.

Virus Infection Symptoms: Vicia faba - mild mosaic and malformation, necrosis.
Diagnostically susceptible host species and symptoms:

  • Pisum sativum - systemic chlorosis, shoot necrosis.
  • Vicia faba - mottle, mosaic and shoot necrosis.

Geographical distribution: Spreads in the African region and the Eurasian region; China, Morocco, and the UK. Found, but with no evidence of spread, in Australia in crops grown from imported seed.

References:

  • Blevings, S. and Stace-Smith, R. (1976). J. gen. Virol. 31: 199.
  • Cockbain, A.J., Cook, S.M. and Bowen, R. (1975). Ann. appl. Biol. 81: 331.
  • Cockbain, A.J., Bowen, R. and Vorra-Urai, S. (1976). Ann. appl. Biol. 84: 321.
  • Gibbs, A.J. and Paul, H.L. (1970). CMI/AAB Descr. Pl. Viruses No. 20, 4 pp.
  • Gibbs, A.J., Giussani-Belli, G. and Smith, H.G. (1968). Ann. appl. Biol. 61: 99.
  • Jones, A.T. (1978). Ann. appl. Biol. 88: 137.
  • Quantz, L. (1953). Phytopath. Z. 20: 421.
  • Randles, J.W. and Dube, A.J. (1977). Australas. Pl. Path. Soc. Newsl. 6: 37.
  • Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.

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